Make your own free website on



M. Kawashima, M. Yamamura, Y. Morita, T. Tanimoto1, M. Kurimoto1, H. Makino

Department of Medicine III, Okayama University Medical School, Okayama 700-8558, 1Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama 702-8006, Japan

Objective: Adult onset Still's disease (AOSD) is a form of polyarthritis associated with systemic manifestations such as high spike fever, sore throat, and an evanescent erythematous salmon-colored rash, and is occasionally complicated by various organ failures. The levels of interleukin-18, a recently identified inflammatory cytokine, in the blood circulation of AOSD was determined.

Methods: Serum samples were obtained from patients with AOSD, rheumatoid arthritis (RA), systemic lupus arthritis (SLE), polymyositis/dermatomyositis (PM/DM), or systemic sclerosis (SSc), or from healthy controls (HC), and their IL-18 levels were measured by a sandwich ELISA. The presence of biological active IL-18 in serum from active AOSD patients were examined by using IL-18-resposive human myelomonocyte cell-lines (KG-1 cells). In addition, serum levels of inflammatory cytokines such as IL-1b, TNF-a, IL-6, and M-CSF were measured by ELISA.

Results: The mean (▒ SD) values of circulating IL-18 were markedly greater in active AOSD patients (n = 14; 80700 ▒ 105100 pg/ml) as compared with patients with either RA (n = 20; 297 ▒ 211 pg/ml), SLE (n = 19; 266 ▒ 113 pg/ml), PM/DM (n = 12; 146 ▒ 44 pg/ml), or SSc (n = 13; 161 ▒ 97 pg/ml), or HC (n = 30; 157 ▒ 69 pg/ml). Serum samples of AOSD patients could stimulate IFN-g production by KG-1 cells, and this activity was blocked by IL-18-specific antibodies; however, their biologically activities of IL-18 were much lower than estimated by ELISA (n = 4; 90 ▒ 74 pg/ml). IL-1b, TNF-a, IL-6, and M-CSF were detectable in serum of AOSD, but these cytokine levels were almost comparable to those of RA. Finally, IL-18 levels were found to be immediately decreased following corticosteroid therapy and correlate with levels of serum ferritin in AOSD patients

Conclusion: These data indicate that IL-18 is circulating at significantly high levels in AOSD patients, in association with their disease activity.

Disclosure: work reported in this abstract was supported by:
Personal funds.

Abstract: 42
November 9, 1998
Poster Session A: Cytokines I
8:00-9:30 am, Hall B1/C

Interleukins: other
Rheumatologic disorders: other



The materials and information on this server are intended for educational and informational purposes only. The materials and information are not intended to replace the services of a trained health professional or to be a substitute for medical advice of physicians and/or other health care professionals. The International Still's Disease Foundation is not engaged in rendering medical or professional medical services. You should consult your physician on specific medical questions, particularly in matters requiring diagnosis or medical attention. The International Still's Disease Foundation makes no representations or warranties with respect to any treatment, action, application medication or preparation by any person following the information offered or provided within this website.  Any information used from other websites was done so with permission from each site, with an exception to those of "public domain", whereas we believe any site without a cited reference was a "public domain site" and for our use.  The International Still's Disease Foundation is a non-profit organization.   This page was last updated on June 13, 2002

Copyrightę 1999-2002 International Still's Disease Foundation